---------- Forwarded message ----------
Date: Fri, 9 Nov 2001 10:01:09 -0500
From: kim parsons <k.m.parsons@abdn.ac.uk>
To: MARMAM@UVVM.UVIC.CA
Subject: Abstract: Dolphin faecal genotyping
Dear Marmamers,
The following paper is now available online through Synergy (http://www.blackwell-synergy.com/Journals/ ), alternatively reprints or copies will be available when I receive them.
Kim Parsons. 2001. Reliable microsatellite genotyping of dolphin DNA from faeces. Molecular Ecology Notes 100: 000-000.
Abstract
Non-invasive samples have proved useful in genotyping studies of free-ranging mammals. However, potential genotyping errors associated with such samples dictate the need for validation studies. This pilot study demonstrates the use of dolphin faeces in multilocus microsatellite genotyping studies. An empirical approach to calculating the rate of genotyping error was applied to data from matched pairs of blood or tissue and faecal samples from both captive and wild bottlenose dolphins. Microsatellite genotypes were assigned to dolphin faecal extracts with greater than 95 percent confidence by using a multiple tube approach, and at least two independent replicate genotypings.
This paper follows on from our previous publication; K.M. Parsons et al. 1999. Amplifying dolphin mitochondrial DNA from faecal plumes. Molecular Ecology 8: 1766 - 1768.
With regards,
Kim Parsons
k.m.parsons@abdn.ac.uk
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Kim M. Parsons
Ph.D. student
University of Aberdeen
Zoology Department
Lighthouse Field Station
George St.
Cromarty
Ross-shire
IV11 8YJ
phone/fax 01381 600548
email k.m.parsons@abdn.ac.uk
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