Abstract: Dolphin faecal genotyping (fwd)

From: Pita Admininstrator (pita@whale.wheelock.edu)
Date: Sun Nov 11 2001 - 08:24:22 EST

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    ---------- Forwarded message ----------
    Date: Fri, 9 Nov 2001 10:01:09 -0500
    From: kim parsons <k.m.parsons@abdn.ac.uk>
    To: MARMAM@UVVM.UVIC.CA
    Subject: Abstract: Dolphin faecal genotyping

    Dear Marmamers,

    The following paper is now available online through Synergy (http://www.blackwell-synergy.com/Journals/ ), alternatively reprints or copies will be available when I receive them.

    Kim Parsons. 2001. Reliable microsatellite genotyping of dolphin DNA from faeces. Molecular Ecology Notes 100: 000-000.

    Abstract
    Non-invasive samples have proved useful in genotyping studies of free-ranging mammals. However, potential genotyping errors associated with such samples dictate the need for validation studies. This pilot study demonstrates the use of dolphin faeces in multilocus microsatellite genotyping studies. An empirical approach to calculating the rate of genotyping error was applied to data from matched pairs of blood or tissue and faecal samples from both captive and wild bottlenose dolphins. Microsatellite genotypes were assigned to dolphin faecal extracts with greater than 95 percent confidence by using a multiple tube approach, and at least two independent replicate genotypings.

    This paper follows on from our previous publication; K.M. Parsons et al. 1999. Amplifying dolphin mitochondrial DNA from faecal plumes. Molecular Ecology 8: 1766 - 1768.

    With regards,

    Kim Parsons
    k.m.parsons@abdn.ac.uk

    .................................................................
    Kim M. Parsons

    Ph.D. student
    University of Aberdeen
    Zoology Department
    Lighthouse Field Station
    George St.
    Cromarty
    Ross-shire
    IV11 8YJ
    phone/fax 01381 600548
    email k.m.parsons@abdn.ac.uk
    .................................................................



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