Subject: Abstract - genetics of Pacific harbor seal (fwd)

Michael Williamson (pita@whale.simmons.edu)
Tue, 6 Aug 1996 15:48:21 -0400 (EDT)

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J. Michael Williamson
   Wheelock College
   Principal Investigator-WhaleNet <http://whale.simmons.edu>
   Associate Professor-Science
voice: 617.734.5200, ext. 256
fax:    617.566.7369

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---------- Forwarded message ----------
Date: Tue, 6 Aug 1996 08:16:11 EST
From: Dagmar_Fertl@smtp.mms.gov
To: Multiple recipients of list MARMAM <MARMAM@UVVM.BITNET>
Subject: abstract - genetics of Pacific harbor seal

     Lamont, M.M., J.T. Vida, J.T. Harvey, S. Jeffries, R. Brown, H.H.
     Huber, R. DeLong, and W.K. Thomas.  1996.  Genetic substructure of the
     Pacific harbor seal (_Phoca vitulina richardsi_) off Washington,
     Oregon, and California.  Marine Mammal Science 12(3): 402-413.

     (Moss Landing Marine Laboratories, P.O. Box 450, Moss Landing, CA
     95039)


     Genetic substructure among groups of Pacific harbor seals, _Phoca
     vitulina richardsi_, along the western coast of the United States was
     investigated using mitochondrial DNA sequences.  Blood and tissue
     samples were removed from 86 seals inhabiting Puget Sound and the
     Pacific coasts of Washington, Oregon, and California.  A 320 base-pair
     segment of the control region was amplified using the polymerase chain
     reaction and directly sequenced.  These data indicated a high level of
     diversity.  Thirty variable sites were found that define 47
     mitochondrial haplotypes.  Among groups of _P. v. richardsi_ sampled,
     5 haplotypes were shared, but most (42) were unique to a locality.
     Haplotypic frequency and an Analysis of Molecular Variance (AMOVA)
     revealed significant differences (P = 0.001) among regions.
     Phylogenetic analysis indicated Puget Sound seals possess unique
     divergent lineages not found in seals from the coasts of Washington,
     Oregon, and California.  These lineages may represent haplotypes from
     north of Washington, which is consistent with late reproductive timing
     of harbor seals from Puget Sound.